The Swine Health Information Center (SHIC) reports positive results from research funded on influenza detection and differentiation diagnostic development; better understanding of influenza control demands sufficient diagnostic capabilities. This has yielded creation of the first 5-Plex RT Influenza PCR for animals that detects and differentiates between Influenza A, B, C and D with one test.
With lessons learned from PED in mind, making sure the pork industry is ready to diagnose emerging diseases is a key part of SHIC's priorities. When the Swine Disease Matrix was created to help prioritize emerging diseases of risk, Influenza C and D Viruses were added and a review of readiness completed. Gaps in preparedness highlighted the need for better influenza diagnostics to help with monitoring and surveillance.
Dr. Xuming Liu, a member of the Molecular Research and Development team at Kansas State Veterinary Diagnostic Laboratory, developed and validated a high-coverage multiplex real-time PCR assay to simultaneously detect and differentiate influenza A, B, C, and D viruses plus a control. This first-of-its-kind assay has 98 to 100 percent coverage of all influenza virus sequences while being sensitive and specific. This is a huge improvement over the previous tests' 40 to 60 percent influenza virus sequence coverage.
The developed assay has wide application for diagnosis, monitoring and surveillance of influenza in swine, bovine, avian, other animals, including humans upon clinical validation.
Wednesady February 14, 2018/ AASV/ United States.