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Predicting in vivo starch digestibility coefficients in newly weaned piglets from in vitro assessment of diets using multivariate analysis

Multivariate modelling could be a successful tool to predict starch digestibility in young pigs.
19 January 2011
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The study was based on correlating a dataset of in vivo mean starch digestibility coefficients obtained in the immediate post-weaning phase of piglets with a range of dietary in vitro variables. The present work presents a model that predicts (R2=0.71) in vivo average starch digestibility coefficients in the half part small-intestinal region of newly weaned piglets fed cereal-based diets using seven in vitro variables describing starch properties that are fundamentally associated with the quality of feed materials, i.e. hydration, structure and amylolytic digestion. The variables were: Rapid Visco Analyser (RVA; measures the viscosity of materials when sheared under defined hydration and temperature regimens); RVA end viscosity; RVA (gelatinisation) peak viscosity; DH (gelatinisation enthalpy that provides an estimate of helical order or degree of crystallinity in starch); water solubility index (WSI; that denotes the amount of soluble polysaccharides released from starch granules to the aqueous phase); grain endogenous amylase (concentration of endogenous a-amylase in cereals, assessed by pasting cereal flours in 25 g of AgNO3, an amylase inhibitor v. water using RVA).

In future the multivariate modelling approach presented could be extended by examining changes in digestibility over time, allowing for investigation of dietary manipulations during critical time windows in the first 2 weeks post-weaning. It should also been remembered that the use of the model is currently limited to diets based on cereals, included in the diet at a rate of 586 g/kg, but did encompass different cereals, processes and piglets demonstrating very different rates of growth.

In its current form, the predictive model does not apply to diets that contain variable amounts of cereals, since most of the in vitro variables used in the equation do not correlate linearly to the amount of starch in diets. It is clear that such an approach could be used to identify not only variation between cereal types and endogenous amylase concentration between different batches of a named cereal, but also the effect these parameters have on starch digestibility within the small intestine of the young piglet.

FJ Doucet, GA White, F Wulfert, SE Hill and J Wiseman, 2010. British Journal of Nutrition. 103: 1309.

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