An accurate diagnosis is sometimes difficult because of the common occurrence of mixed infections, the compounding effects of environment and nutrition and the difficulties experienced in isolating and identifying all the possible pathogenic agents involved.
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The main aspect that cannot be forgotten is the complete approach necessary to evaluate all possibilities. In that sense, samples have to be collected and tested to all possible agents that might cause diarrhea.
The ideal sample for diagnostic of neonatal enteric pathogens is the submission of live piglets.