TwitterLinkedinWhatsAppTelegramTelegram
0
Read this article in:

First detection of antibodies against African Swine Fever virus in faeces samples

This study assessed the feasibility of detecting antibodies against ASFV in faeces for later implementation in surveillance and control programmes.

18 November 2015
TwitterLinkedinWhatsAppTelegramTelegram
0

Wild boar populations have been recently assigned an essential role in the spread of African swine fever virus (ASFV) to European countries. Therefore, effective surveillance and monitoring of wild boar populations is required, but sampling wild boar is logistically challenging and expensive.

This study assessed the feasibility of detecting antibodies against ASFV in faeces for later implementation in surveillance and control programmes. Two groups of pigs were experimentally infected with an attenuated ASFV isolate Ken05, and blood, oral fluid and faecal samples were tested for the presence of viral DNA using quantitative real-time polymerase chain reaction (qPCR) to monitor infection progress. Faecal samples were analysed using two indirect enzyme-linked immunosorbent assays (ELISAs) based on semipurified viral protein (vp) 72 or purified recombinant vp30 expressed in mammalian cells. Faecal samples from 9 of 10 pigs with non-haemorrhagic diarrhoea tested positive for antibodies against ASFV using the two ELISA tests that showed a positive correlation. The serum sample results from the two indirect ELISAs were compared against results from the reference ELISA technique and the immunoperoxidase test. Our findings indicate the feasibility of faecal sampling for detecting anti-ASFV antibodies, which may provide a practical non-invasive alternative for sampling wild boar populations.

In conclusion, the application of these ELISA tests to faecal field samples could be particularly useful to screen for the presence of ASF in field conditions.

E. Nieto-Pelegrín, B. Rivera-Arroyo and J. M. Sánchez-Vizcaíno. First Detection of Antibodies Against African Swine Fever Virus in Faeces Samples. Transboundary and Emerging Diseases. Volume 62, Issue 6, pages 594–602, December 2015. DOI: 10.1111/tbed.12429

Article Comments

This area is not intended to be a place to consult authors about their articles, but rather a place for open discussion among pig333.com users.
Leave a new Comment

Access restricted to 333 users. In order to post a comment you must be logged in.

Related products in the shop

The shop specialized in the pig sector
Advice and technical service
More than 120 brands and manufacturers

Related articles

Advance in the fight against ASF

29-Feb-2016
The research is focused on one of the pig genes associated with African Swine Fever Virus infection called RELA. The gene causes the immune system to overreact with devastating effects.