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Since circoviruses commonly occur in the pig populations and there is a correlation between the severity of the disease and the viral load in the organs and blood, it is important not only to detect PCV2 but also to determine the quantitative aspects of viral load. The PriProET real-time PCR assay described in this study was tested on various virus strains and clinical forms of PMWS in order to investigate any correlation between the clinical signs and viral loads in different organs.
The data obtained in this study correlate with those described earlier; namely, the viral load in 1 ml plasma and in 500 ng tissue DNA exceeds 107 copies in the case of PMWS.
The results indicate that the new assay provides a specific, sensitive and robust tool for the improved detection and quantification of PCV2.
Bálint A, Tenk M, Deim Z, Rasmussen TB, Uttenthal A, Cságola A, Tuboly T, Farsang A, Fossum C, Timmusk S, Berg M, Belák S. Development of Primer-Probe Energy Transfer real-time PCR for the detection and quantification of porcine circovirus type 2. Acta Vet Hung. 2009 Sep;57(3):441-52.
