PCR can detect the virus in virtually any material but, does it mean the sample contains the virus and it is infectious? Is any PCR test valid for any strain?
Warsaw University of Life Sciences. Poland
Prof. Tomasz Stadejek graduated from the Faculty of Veterinary Medicine of the University of Life Sciences in Lublin, Poland in 1990. In 1991-2011 he was employed at the Department of Swine Diseases of the National Veterinary Research Institute in Pulawy, Poland. Since 2012 he is a full professor at the Faculty of Veterinary Medicine of the Warsaw University of Life Sciences in Warsaw, Poland. He obtained his PhD in 1996 and DSc in 2002. In 2007 Prof. Stadejek was nominated by the World Organization for Animal Health (O.I.E.) as an expert for PRRS. He is a member of the Arterivirus Study Group of the International Committee on Taxonomy of Viruses. He is a diplomate of the European College for Porcine Health Management and in 2009-2012 he served the secretary of the college. The primary areas of interest are diagnosis, epidemiology and control of porcine virus diseases. Current research is focused on the evolution and genetic and antigenic diversity of European genotype PRRSV and its impact on sensitivity and specificity of diagnostic methods and on efficacy of methods to control the disease.
Updated CV 07-Oct-2013
Currently, no single RT-PCR assay could be recommended as a universal method allowing for detection of all PRRSV strains with optimal sensitivity.
The great genetic diversity of East European strains has practical implications, the sensitivity of some of the diagnostic PCR methods can be compromised and the efficacy of the current PRRSV vaccines against the East European genetic variants still needs to be elucidated.
The ASF situation in Poland (19 cases and 2 outbreaks in 4 municipalities) seems to be very different from the countries east from Poland: it can be concluded that among wild boar in 4 municipalities the course of the disease is endemic rather than epidemic.
A comprehensive picture of viral variation at the beginning of control and elimination projects is critical for effective monitoring of progress and effectiveness of implementation procedures, and for identification of new virus introductions to farms and to the region.
After the clinical and serological evaluation it is decided to vaccinate against PRRSV, with which we achieve a gradual improvement in the health status on the farm. Some months later, a severe cough appears in the fattening pigs that later also appears in the nursery and in the sows.