Researchers of CReSA and the Centro Nacional de Sanidad Agropecuaria (CENSA) have developed a real-time PCR system for the detection and differentiation of porcine circovirus 2 (PCV-2), pseudorabies virus (PRV1), porcine parvovirus (PPV) and swine torque teno viruses 1 (TTSuV1) and 2 (TTSuV2).
Real-time PCR techniques have improved the diagnosis of important viral livestock diseases and have become established scientific tools in veterinary virology and disease control. Particularly, the SYBR Green I-based real-time assay has proven to be one of the most effective tools in the rapid and differential detection of a variety of viral pathogens. The SYBR Green I assay is considered a flexible and cost-effective approach that, coupled with melting curve analysis, ensures reaction specificity along with multiplex detection of distinct targets.
The present study was undertaken to develop and evaluate a multiple SYBR Green I-based real-time PCR system for the detection and differentiation of PCV-2, PRV, PPV, TTSuV1 and TTSuV2 in a single run. The analytical and diagnostic performances obtained from the assessment of field samples, as well as the repeatability of the tests in the system; indicate the usefulness of this tool for laboratory diagnosis and epidemiological investigations.
Thursday May 2, 2013/ CReSA/ Spain.