Supplemental sodium butyrate stimulates different gastric cells in weaned pigs

The use of sodium butyrate just after weaning may help to stimulate gastric mucosal maturation
Sunday 19 April 2009 (9 years 11 months ago)
The objective of the present work was to study the effect of sodium butyrate (SB) orally supplemented during the suckling and/or post-weaning period on various aspects of stomach morphology and physiology after the post-weaning period.

The present experiment involved two different periods: 1) the suckling period from 4 d after birth until weaning at 28 d of age; and 2) the post-weaning period from d 29 to 40 (day of slaughter). Two different factors were studied: butyrate before weaning (BE) and butyrate after weaning (AF). The combination of these factors were defined as: pigs never supplemented with SB (CC), pigs supplemented only before weaning (BC), pigs supplemented only after weaning (CB) and pigs supplemented both before and after weaning (BB). A total of 32 piglets of same birth weights from 4 different litters were used. Milk intake for each animal was estimated during the suckling period from its growth rate. Two solutions were prepared for oral administration during the suckling period: 1) saline solution (9 g NaCl/L) for the control groups (CC and CB); and 2) SB solution (60 g/L) dissolved in saline solution for the SB groups (BC and BB). For the CB and BB groups, SB was introduced (3 g/kg) in 1 of these starter diets by replacing corn starch. The piglets from 2 litters were randomly killed 4 h after the last meal on d 11 and those from the remaining 2 litters on d 12 after weaning (d 39-40 of age).The number of parietal cells immunostained for H1/K1-ATPase, gastric endocrine cells immunostained for chromogranin A and somatostatin (SST) in the oxyntic mucosa, and gastrin-secreting cells in the pyloric mucosa was studied. Gastric muscularis and mucosa thickness were also measured. Expressions of the H1/K1-ATPase and SST type 2 receptor (SSTR2) genes in the oxyntic mucosa and of the gastrin gene in the pyloric mucosa were evaluated by real-time RT-PCR. The SB inclusion increased the number of parietal cells per gland regardless of the period of administration (P<0.05). SB addition after, but not before, weaning increased the number of enteroendocrine and SST-positive cells (P<0.01) and tended to increase gastrin mRNA (P<0.09). There was an interaction between the 2 periods of SB treatment for the expression of H/K-ATPase and SSTR2 genes (P<0.05). Butyrate intake after weaning increased gastric mucosa thickness (P<0.05) but not muscularis.

It is concluded that the SB orally used at a low dose affected gastric morphology and function, presumably in relationship with its action on mucosal maturation and differentiation.

M Mazzoni, M Le Gall, S de Filippi, L Minieri, P Trevisi, J Wolinski, G Lalatta-Costerbosa, JL Lall├Ęs, P Guilloteau and P Bosi. 2008. Journal of Nutrition. 138: 295-299.

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