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Detection of a novel circular single-stranded DNA virus from porcine feces in Korea

This was the first observation in Korea, bringing about the possibility that this virus may be prevalent in porcine farms.

Wednesday 4 December 2013 (4 years 10 months 17 days ago)
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Circular ssDNA viruses can infect a wide range of hosts such as chimpanzees, bovines, bats, pigs and so on, imposing that they can also be transmitted to human indirectly or directly. Even though they share common characteristics in terms of organization of genome or structures, their sequences of genome tend to be variable, showing different phylogenetic patterns. Recently, a novel bovine stool associated circular ssDNA viruse has beenisolated in Korea. Later, PigSCV and PoSCV have been discovered in Germany and New Zealand, respectively. Therefore, it was assumed that a novel circular ssDNA virus might be able to be identified from porcine feces in Korea. So, for the first time, a large number of porcine fecal samplesfrom Korean farms have been screened for the detection of PoSCV during the six-month period from July 2012 to December 2012.

Total 233 stool samples of pigs with diarrhea were collected from July 2012 to December 2012. They varied by ages, times, or regions. DNA extraction of these samples were conducted. DNA samples were analyzed using a nested PCR assay.

The results indicated the presence of the nucleic acid of PoSCV in porcine fecal samples. The total detection rate was around 62% (144/233). No significant differences in the prevalence of PoSCV were found among age groups and regions. However, the detection rates in November and December were approximately 2 times as low as those in July and August.

The detection rate of PoSCV in porcine stool samples in Korea during the six months of surveyance was approximately 60%. This was the first observation in Korea, bringing about the possibility that this virus may be prevalent in porcine farms. The results showed that both ages and regions of pigs were not highly relevant to the occurrence of PoSCV, while seasons may be associated with. Besides, the nested PCR assay could be considered as a reliable method for the identification of PoSCV by improving the sensitivity. Further studies on full-sequencing of the positive samples are suggested, and difference in the sequence of these strains and strains detected worldwide would be phylogenetically analyzed.

Hee C. Chung, A R. Kim, Eun O. Kim, Hye K. Kim, Van G. Nguyen, Min K. Choi, Hye J. Yang, Jung A. Kim, Bong K. Park. The 6th Asian Pig Veterinary Society Congress. 2013. OR52

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