
Laboratory diagnostics: Influenza A virus (IAV)
What laboratory diagnostic methods can I use to diagnose influenza A? Which one should I choose according to the situation? How do I interpret the results?
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List of the most important diseases and conditions in pigs. Symptoms, causes, diagnosis, control and prevention of each disease are described. Some of the treatments mentioned may be prohibited in some countries. Information on all diseases to be completed in the coming days.
Influenza is a respiratory disease of high importance due to its fast transmission and zoonotic potential.
Porcine influenza is caused by several Influenza Type A viruses closely related, characterized to have the ability to change the antigenic structure and create new strains.
Each serotype is identified through surface proteins called "H" and "N". The three most common serotypes affecting pigs are H1 N1, H1 N2 and H3 N2. There are also different strains of these serotypes that have different pathogenicity and no crossed protection.
The incubation period is very short, 12 to 48 hours. It affects the respiratory system. The virus can infect a farm in few days. This high transmission is almost the main feature helping in the diagnosis.
There are three important periods in which the disease can cause infertility, and all of them are caused by fever and its consequences depending on the pregnancy stage because the virus is not systemic and does not cross the placenta. Thus, fetuses are not infected.
In big farms, influenza can end up being endemic with intermittent outbreaks of clinical symptoms and infertility. Different strains can consecutively affect one farm.
Sows
When the virus enters a farm for the first time, 2 or 3 animals show symptoms of disease during the first 2 days, followed by:
In the rest of the farm:
Lactating piglets
Weaners and growers
Acute Disease
Endemic Disease
Influenza can be introduced by:
In the acute disease, a reliable diagnosis can be done based on clinical signs, due to the fact that there are no other diseases with such a dramatically quick transmission and clinical effects. Blood samples taken from sows at the beginning of the disease and 2 to 3 weeks after, show an increase in antibody titters. Influenza virus can be identified from nasal swabs analyzed by PCR. Oral fluids can also be used, with good results.
However, the endemic disease can be difficult to differentiate form other viral diseases. PRRS and Aujeszky’s disease must be considered.
Sequencing is critical to establish the vaccine strain.

What laboratory diagnostic methods can I use to diagnose influenza A? Which one should I choose according to the situation? How do I interpret the results?

In this last article of the series, we discuss management strategies and the vaccines available to control swine influenza.

The clinical picture associated with swine influenza is often complicated by temperature fluctuations, other viruses, or bacteria. In part two of this series, we look at the clinical signs of this disease.
















