Mariamawit Mohammed

Iowa State University - ISU - Research assistant - United States Author

5 Followers 2 Articles

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Research assistant

Iowa State University - ISU

2024 - Present

Studies

PhD

Iowa State University - ISU

Present

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Mariamawit Mohammed

5 Followers 2 Articles

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Figure 1: Representation of a recombination event generating a new virus. The region before and after the points where the two lines cross denote the portion of genomes received from the two parental viruses. Genome regions are shown at the top.

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The implications of the PRRSV recombination dilemma

PRRS control measures also reduce the opportunities for different wild-type viruses to recombine and create more aggressive strains.

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Figure 1: Schematic representation of a PRRSV whole genome (GenBank U87392) and the target regions for different diagnostic assays. In the United States, RT-PCR assays to detect modified live virus (MLV) vaccine-like viruses target the nsp2 region, and CLAMP sequencing to block amplification of MLV vaccine viruses during Sanger sequencing targets the ORF5 gene.

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PRRS Molecular diagnostics: when sequencing only 4% is not enough

Genetic mutation and evolution of PRRSV can occur across all genes. Unlike ORF5, which only represents about 4% of the PRRSV genome, NGS can recover a whole PRRSV genome to be used in epidemiological investigations.

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02-Jun-2025sonu-kumar_1hack

Mariamawit Mohammed

C. Vitae

Studies

The implications of the PRRSV recombination dilemma

PRRS Molecular diagnostics: when sequencing only 4% is not enough

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