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Fundamentals and mechanisms of the ELISA test

High sensitivity, specificity, and an affordable cost make ELISA an indispensable tool in laboratory routines. This infographic explains the mechanisms, components, and main variations of this assay in a visual and objective way.

The Enzyme-Linked Immunosorbent Assay (ELISA) is a method that relies on the specific binding between antigens and antibodies, followed by enzymatic detection, typically through a colorimetric reaction. This technique enables the direct or indirect quantification of analytes with high sensitivity and specificity. It is widely used in the diagnosis of infectious diseases, biomarker assessment, hormone testing, and cytokine assays.

This infographic provides an objective overview of the mechanisms of action, structural components, and main variants of this immunoassay based on antigen-antibody binding.

The information used to create this infographic has been extracted from the article "ELISA assay as a diagnostic tool (1/2): Basics and uses" by Alejandro Ramirez.

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