In recent years, CRISPR technology has emerged as a transformative tool in the field of biology. CRISPR library screening is a high-throughput screening method based on the CRISPR/Cas9 gene editing system, designed to interfere with the genome using a large-scale sgRNA library to discover new drug targets associated with specific phenotypes or biological processes. This technology employs precisely designed sgRNA sequences introduced into target cells or organisms, facilitating screening under drug pressure or cell sorting conditions to identify genes closely associated with the target phenoty